Large-scale production of tetanus toxoid.

was able to obtain large volumes of high potency material by batch cultivation in tanks, but did not report any yields. was sterilized at 118 to 122 C for 1 hr, cooled rapidly to 36 to 39 C, and inoculated immediately. Seed cultures of Clostridium tetani (Massachusetts strain) were maintained at -70 C in 1.0-ml amounts in glass ampoules. One or four of these ampoules were thawed and inoculated into 200 to 300 ml of Brewer's thioglycolate broth contained in 500-ml bottles. After incubation at 37 C for 24 to 30 hr, these cultures were used to inoculate the tank. Tank cultures were incubated for 7 to 8 days